Influence of phytochemicals in piper betle linn leaf extract on wound healing

 http://repository.vnu.edu.vn/handle/VNU_123/26846

Background

Wound healing has being extensively investigated over the world.

Healing impairment is caused by many reasons including increasing of free-radicals-mediated damage, delaying in granulation tissue formation, reducing in angiogenesis and decreasing in collagen reorganization. 

These facts consequently lead to chronic wound healing.

Piper betle Linn (Betle) leaves have been folklore used as an ingredient of drugs for cutaneous wound treatment.

However, the effect of betle leaf on wound healing is not yet well elucidated.

In this study, we aimed to investigate the healing efficacy of methanol leaf extract of Piper betle Linn on proliferation of fibroblast NIH3T3 cells as well as full-thickness burn and excision wounds in swiss mice. 

Methods

Scratch wound healing assays were conducted to examine the effects of betle leaf extract on healing activity of fibroblast cells.

Burn and excision wounds on swiss mouse skins were created for investigating the wound healing progress caused by the betle leaf extract.

Malondialdehyde (MDA) was also evaluated to examine the products of lipid hydroperoxide (LPO) under conditions of with or without betle leaf extract treatment.

Results

The results of this study showed that Piper betle Linn leaf extract in methanol increased proliferation of NIH3T3 cells and promoted wound healing in vitro and in vivo with both burn wound and excision wound models.

In addition, this extract significant decreased level of malondialdehyde (MDA) in liver of treated-mice compared with that in non-treated mice.

Conclusions

Our results suggest that Piper betle Linn can be used as an ingredient in developing natural origin drugs for treatment of cutaneous wounds

Title: 

	Influence of phytochemicals in piper betle linn leaf extract on wound healing
Authors:	Le, Thi Lien Nguyen, Thi Tho Do, Minh Ha Pham, Luong Hang Phan, Tuan Nghia Nguyen, Dinh Thang
Keywords:	Piper betle Linn Leaf extract Wound healing Malondialdehyde (MDA) Fibroblast (NIH3T3)
Issue Date:	2015
Publisher:	Đại học Quốc gia Hà Nội
Citation:	ISIKNOWLEDGE
Abstract:	Background: Wound healing has being extensively investigated over the world. Healing impairment is caused by many reasons including increasing of free-radicals-mediated damage, delaying in granulation tissue formation, reducing in angiogenesis and decreasing in collagen reorganization. These facts consequently lead to chronic wound healing. Piper betle Linn (Betle) leaves have been folklore used as an ingredient of drugs for cutaneous wound treatment. However, the effect of betle leaf on wound healing is not yet well elucidated. In this study, we aimed to investigate the healing efficacy of methanol leaf extract of Piper betle Linn on proliferation of fibroblast NIH3T3 cells as well as full-thickness burn and excision wounds in swiss mice. Methods: Scratch wound healing assays were conducted to examine the effects of betle leaf extract on healing activity of fibroblast cells. Burn and excision wounds on swiss mouse skins were created for investigating the wound healing progress caused by the betle leaf extract. Malondialdehyde (MDA) was also evaluated to examine the products of lipid hydroperoxide (LPO) under conditions of with or without betle leaf extract treatment. Results: The results of this study showed that Piper betle Linn leaf extract in methanol increased proliferation of NIH3T3 cells and promoted wound healing in vitro and in vivo with both burn wound and excision wound models. In addition, this extract significant decreased level of malondialdehyde (MDA) in liver of treated-mice compared with that in non-treated mice. Conclusions: Our results suggest that Piper betle Linn can be used as an ingredient in developing natural origin drugs for treatment of cutaneous wounds.
Description:	BURNS & TRAUMA Volume: 3 Article Number: 23 Published: DEC 1 2015 ; TNS05539 ; 8p.
URI:	http://repository.vnu.edu.vn/handle/VNU_123/26846
Appears in Collections:	Bài báo của ĐHQGHN trong Web of Science

Files in This Item: